isa 2011b t23498 Search Results


isa 2011b t23498  (TargetMol)
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TargetMol isa 2011b t23498
A ) sgCTL control, TBC1D19 -/- , and TTLL1 -/- cells were serum starved for 24 hr, and immuno-stained with antibodies against acetylated tubulin and INPP5E. ≥ 80 cilia were counted per sample in three independent experiments. Error bars, S.D. **** p ≤ 0.0001. Representative images were shown for each cell line. Scale bar, 1µm. ns, not significant. B ) sgRNA control and TBC1D19 knockout cells were serum starved for 48 hr, re-fed with serum-containing medium, fixed after 30 min, 2 hr, or 3 hr, and immuno-stained with GT335 antibody. ≥ 70 cells were counted per sample in two independent experiments. Error bars, S.D. *p ≤ 0.05, **p ≤ 0.01. C ) Ciliary resorption rate (or percentage reduction in ciliation) after serum addition was calculated based on data in panel B. Error bars, S.D. *p ≤ 0.05. D ) sgRNA control and TTLL1 knockout cells were treated and stained as in panel B. N ≥ 100 cells were counted per sample in two independent experiments. Error bars, S.D. ns, not significant. E ) sgRNA control and TBC1D19 knockout cells were serum starved for 24 hr, simultaneously treated with DMSO, 1μM LY-294002 (LY), 1μM or 10 μM UNC3230 (UNC), or 1μM or 10 μM <t>ISA-2011B</t> (ISA), as indicated, and immuno-stained with GT335. ≥ 70 cells were counted per sample in two independent experiments. Error bars, S.D. *p ≤ 0.05, **p ≤ 0.01. ns, not significant.
Isa 2011b T23498, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A ) sgCTL control, TBC1D19 -/- , and TTLL1 -/- cells were serum starved for 24 hr, and immuno-stained with antibodies against acetylated tubulin and INPP5E. ≥ 80 cilia were counted per sample in three independent experiments. Error bars, S.D. **** p ≤ 0.0001. Representative images were shown for each cell line. Scale bar, 1µm. ns, not significant. B ) sgRNA control and TBC1D19 knockout cells were serum starved for 48 hr, re-fed with serum-containing medium, fixed after 30 min, 2 hr, or 3 hr, and immuno-stained with GT335 antibody. ≥ 70 cells were counted per sample in two independent experiments. Error bars, S.D. *p ≤ 0.05, **p ≤ 0.01. C ) Ciliary resorption rate (or percentage reduction in ciliation) after serum addition was calculated based on data in panel B. Error bars, S.D. *p ≤ 0.05. D ) sgRNA control and TTLL1 knockout cells were treated and stained as in panel B. N ≥ 100 cells were counted per sample in two independent experiments. Error bars, S.D. ns, not significant. E ) sgRNA control and TBC1D19 knockout cells were serum starved for 24 hr, simultaneously treated with DMSO, 1μM LY-294002 (LY), 1μM or 10 μM UNC3230 (UNC), or 1μM or 10 μM ISA-2011B (ISA), as indicated, and immuno-stained with GT335. ≥ 70 cells were counted per sample in two independent experiments. Error bars, S.D. *p ≤ 0.05, **p ≤ 0.01. ns, not significant.

Journal: bioRxiv

Article Title: The tubulin poly-glutamylase complex, TPGC, is required for phosphatidyl inositol homeostasis and cilium assembly and maintenance

doi: 10.1101/2025.03.03.641315

Figure Lengend Snippet: A ) sgCTL control, TBC1D19 -/- , and TTLL1 -/- cells were serum starved for 24 hr, and immuno-stained with antibodies against acetylated tubulin and INPP5E. ≥ 80 cilia were counted per sample in three independent experiments. Error bars, S.D. **** p ≤ 0.0001. Representative images were shown for each cell line. Scale bar, 1µm. ns, not significant. B ) sgRNA control and TBC1D19 knockout cells were serum starved for 48 hr, re-fed with serum-containing medium, fixed after 30 min, 2 hr, or 3 hr, and immuno-stained with GT335 antibody. ≥ 70 cells were counted per sample in two independent experiments. Error bars, S.D. *p ≤ 0.05, **p ≤ 0.01. C ) Ciliary resorption rate (or percentage reduction in ciliation) after serum addition was calculated based on data in panel B. Error bars, S.D. *p ≤ 0.05. D ) sgRNA control and TTLL1 knockout cells were treated and stained as in panel B. N ≥ 100 cells were counted per sample in two independent experiments. Error bars, S.D. ns, not significant. E ) sgRNA control and TBC1D19 knockout cells were serum starved for 24 hr, simultaneously treated with DMSO, 1μM LY-294002 (LY), 1μM or 10 μM UNC3230 (UNC), or 1μM or 10 μM ISA-2011B (ISA), as indicated, and immuno-stained with GT335. ≥ 70 cells were counted per sample in two independent experiments. Error bars, S.D. *p ≤ 0.05, **p ≤ 0.01. ns, not significant.

Article Snippet: UNC3230 (T15597) and ISA-2011B (T23498) were purchased from TargetMol.

Techniques: Control, Staining, Knock-Out